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HOME > Kosin Med J > Volume 31(1); 2016 > Article
Case Report
Identification of streptococcus dysgalactiae subsp. equisimilis from septic knee by 16S rRNA gene sequencing
Ji Sang Park1, Hye Won Kim1, Jeong Don Chae2, Jin Wuk Hur1, Yoon Seok Park1, Min Seok Yoo1, Hee Yoon1
Kosin Medical Journal 2016;31(1):79-85.
DOI: https://doi.org/10.7180/kmj.2016.31.1.79
Published online: February 4, 2016

1Department of Internal Medicine, Eulji General hospital, College of Medicine, Eulji University, Seoul, Korea

2Department of Laboratory Medicine, Eulji General hospital, College of Medicine, Eulji University, Seoul, Korea

Corresponding Author: Jeong Don Chae, Department of Laboratory Medicine, Eulji General Hospital, College of Medicine, Eulji University, 202, Dosan-daero, Gangnam-gu, Seoul, 06047, Korea Tel: +82-2-970-8324 Fax: +82-2-970-8534 Email: jdchae@eulji.ac.kr
• Received: July 30, 2015   • Revised: September 1, 2015   • Accepted: September 9, 2015

Copyright © 2016 Kosin University School of Medicine Proceedings

This is an Open Access article distributed under the terms of the Creative Commons Attribution Non-Commercial License (http://creativecommons.org/licenses/by-nc/3.0/) which permits unrestricted non-commercial use, distribution, and reproduction in any medium, provided the original work is properly cited.

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  • Septic arthritis is the infection of a joint by an infectious agent, leading to arthritis. It is therefore important to identify and treat the correct bacteria in septic arthritis. However, accurate identification of bacteria by conventional methods is difficult because of the distinct biochemical characteristics of individual bacteria. This case report aims at assessing septic arthritis caused by Streptococcus dysgalactiaesubsp. equisimilis(SDSE) using nucleotide sequences and discusses the associated treatment. Here, Streptococcus agalactiaewas determined to be the causative bacteria for septic arthritis in a 77 year-old woman using the conventional method of hemolysis pattern interpretation and morphology. However, nucleotide sequence analysis of 16S ribosomal RNA revealed that SDSE was the causative strain. 16S rRNA gene sequencing can correctly identify bacteria strains that are difficult to be identified by traditional method, and this correct identification can provide patients with the opportunity for adequate treatment using the proper antibiotics.
  • Keywords: Key Words; Equisimilis; Infectious arthritis; Streptococcus dysgalactiaesubsp.; 16S rRNA gene sequencing
Fig. 1.
Irregular thick enhancement of synovium with joint effusion (arrows) on an Axial T1-enhanced magnetic resonance (MR) image of the left knee.
kmj-31-79f1.jpg
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  • 7. Park MJ, Eun IS, Jung CY, Ko YC, Kim YJ, Kim CK, et al. Streptococcus dysgalactiae subspecies dysgalactiae infection after total knee arthro-plasty: a case report. Knee Surg Relat Res 2012;24:120–3.ArticlePubMedPMC

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        Identification of streptococcus dysgalactiae subsp. equisimilis from septic knee by 16S rRNA gene sequencing
        Kosin Med J. 2016;31(1):79-85.   Published online February 4, 2016
        DOI: https://doi.org/10.7180/kmj.2016.31.1.79
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      Identification of streptococcus dysgalactiae subsp. equisimilis from septic knee by 16S rRNA gene sequencing
      Image
      Fig. 1. Irregular thick enhancement of synovium with joint effusion (arrows) on an Axial T1-enhanced magnetic resonance (MR) image of the left knee.

      Fig. 1.

      Identification of streptococcus dysgalactiae subsp. equisimilis from septic knee by 16S rRNA gene sequencing
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