Fig. 1These photos show parts of surgical resection (arrow head) and histological biopsy (arrow A, B and C)
Fig. 2The diameter of mouse tail was measured with rod ruler by photograph.
Fig. 3Tail volume changes following removal of skin and subcutaneous tissues. The study group displayed nearly twice volume at post - 18 days.
Fig. 4Lymphoscintigraphy was performed after the intradermal injection of 100 uCi/0.02 ml of filtered 99mTc - phytate. Dynamic and static images were acquired using a low energy general purpose collimator in a microSPECT INFINIA gamma camera. The renal nodes (arrow head) were visualized significantly in control group (A). But, in study group (B), renal nodes were visualized 30 minute after injection and dermal back flow (arrow) was observed.
Fig. 5Normal tail skin harvested 18 mm from the base of the tail shows thin epidermis and no dilated vessels in dermis (5-A, H&E stain × 40). Some dilated vessels in dermis (arrows) are seen at the groups of 2 and 4 weeks, respectively (5-B and 5-C, H&E stain × 40).
Fig. 6Positive reaction of endothelial cell in dilated vessel is noted by lymphatic vessel hyaluronan receptor - 1 (LYVE - 1) stain. (× 400).
Table 1Change of Diameter on the Mouse Tail