Kosin Medical Journal

Omega-3 fatty acids upregulate Nrf2 expression and attenuate apoptosis, inflammation, and fibrosis in a rat model of cyclosporine-induced nephropathy: Ji Young Lee, Young Ki Son, Mi Hwa Lee, Su Mi Lee, Seong Eun Kim, Won Suk An; Kosin Med J. 2023;38(3):184-192. Published online July 26, 2023; DOI: https://doi.org/10.7180/kmj.23.112

171 View
9 Download

Abstract PDF PubReader ePub Crossref - TDM: Background
Cyclosporine A (CsA)-induced kidney injury is characterized by renal impairment with inflammatory cell infiltrations, apoptosis, fibrosis, and hypoxic injury. It is not clear whether omega-3 fatty acids (O-3 FAs), which have anti-inflammatory and antioxidant roles, affect nuclear factor erythroid 2-related factor 2 (Nrf2) expression. The aim of this study was to investigate whether O-3 FAs affect Nrf2 expression and exert anti-inflammatory, anti-apoptotic, and anti-fibrotic effects in CsA-induced nephropathy.
Methods
Male Sprague-Dawley rats were divided into control, CsA-treated, and CsA-treated with O-3 FA groups. Nrf2 expression was measured by Western blots and immunohistochemical staining.
Results
Kidney function was impaired in the CsA-treated rats compared to the controls. Caspase-3 and caspase-7 were activated in the CsA-treated group, and the Bax/Bcl2 ratio was higher. O-3 FAs attenuated these apoptosis-related changes. ED-1 and inhibition of kappa B (IĸB) protein expression were significantly upregulated in the CsA-treated group. Compared to the control group, O-3 FA supplementation attenuated the increased expression of ED-1 and IĸB related to inflammation. Smad2/3, Smad4, and transforming growth factor-β1 were activated in the CsA group, and O-3 FA treatment prevented these changes related to renal fibrosis. The expression of Nrf2 was reduced in CsA-treated rats, but Nrf-2 was increased by O-3 FA treatment.
Conclusions
We suggest that Nrf2 is a potential mediator induced by O-3 FA supplementation and that it attenuates pro-inflammatory pathways, fibrotic processes, and apoptosis. Further studies are needed to elucidate the crosstalk between Nrf2 expression and signals related to O-3 FA treatment.

Transcriptome analysis of the pathogenic ciliate Miamiensis avidus after hydrogen peroxide treatment: Hyunsu Kim, A-Reum Lee, Kyung-Yoon Jeon, Eun-Ji Ko, Hee-Jae Cha, Mee Sun Ock; Kosin Med J. 2022;37(1):52-60. Published online March 11, 2022; DOI: https://doi.org/10.7180/kmj.21.040

1,459 View
23 Download

Abstract PDF PubReader ePub Crossref - TDM: Background
The scuticociliate Miamiensis avidus is a highly pathogenic ciliate responsible for serious damage to various organs of aquaculture fish. In particular, the olive flounder aquaculture industry is suffering massive losses due to M. avidus infection. Hydrogen peroxide (H2O2) is one of the most widely used chemicals for scuticociliate treatment. Despite the superior killing effect of H2O2, studies on transcription levels and gene expression changes after H2O2 treatment are limited. We conducted an mRNA transcriptome analysis to compare the expressed gene (DEG) profiles between the ciliate and cyst-like stages of M. avidus after H2O2 treatment.
Methods
We applied differentially expressed gene profiling to identify DEGs during the ciliate and cyst-like stages of M. avidus.
Results
There were 5,967 DEGs among the 9,075 transcripts identified, and 50 of these DEGs were significantly different (p<0.05). Among these, 21 DEGs were upregulated and 29 were downregulated in the cyst-like stage. The most significantly upregulated genes during the change to the cyst-like stage were cytochrome c oxidase genes. Genes related to the calcium channel were also highly upregulated.
Conclusion
The significant upregulation of cytochrome c gene expression and cytosolic calcium ion channel-related gene expression after H2O2 treatment suggests that ciliate mortality occurred through apoptosis. The formation of the cyst-like stage is considered a temporary form during the process of apoptosis. Information on the gene expression profile of M. avidus in response to H2O2 is expected to contribute to the understanding of the mechanism of action of therapeutic agents against this pathogen.

Effect of Docosahexaenoic Acid (DHA) on Breast Cancer Cells: Sun-yong Hwang, Tae-Hee Kim, Hae-Hyeog Lee, Heung Yeol Kim, Juhyun Seo; Kosin Med J. 2015;30(2):103-107. Published online January 20, 2015; DOI: https://doi.org/10.7180/kmj.2015.30.2.103

794 View
5 Download
2 Citations

Abstract PDF PubReader ePub Crossref - TDM

Abstract

Today, many materials as drug are developed having various prominent function in order to treatment of disease or cancer. Among these materials, especially docosahexaenoic acid (DHA), main constituents of omega-3 fatty acid, has a lot of beneficial and natural effects, so it has been known as anticancer material especially breast cancer. Breast cancer is disease taking high occurrence level among feminine diseases. DHA has anticancer effects on breast cancer cell, representatively inducing apoptosis, inhibiting proliferation or metastasis. Main effect of DHA on breast cancer cell is apoptosis inducing, which has mechanism that treated DHA causes lipid peroxidation increasing reactive oxygen species (ROS) level and it activates caspase 8 and caspase 9 so activated caspase occurs apoptosis. Cell lines of breast cancer are MDA-MB-231, MCF-7, SK-BR-3, T47D and ZR75. Especially this article uses the MCF-7 cell line at experiment of anti-proliferation by DHA, the MDA-MB-231 cell line at experiment of anti-metastasis by DHA, because that cell line has specialized metastasis activity. Therefore, this paper discusses the effects of natural material DHA as drug of breast cancer.

Citations

Citations to this article as recorded by

The Function and Mechanism of Lipid Molecules and Their Roles in The Diagnosis and Prognosis of Breast Cancer
Rui Guo, Yu Chen, Heather Borgard, Mayumi Jijiwa, Masaki Nasu, Min He, Youping Deng
Molecules.2020; 25(20): 4864. CrossRef
Plasma metabolites as possible biomarkers for diagnosis of breast cancer
Jiwon Park, Yumi Shin, Tae Hyun Kim, Dong-Hyun Kim, Anbok Lee, Mohana Krishna Reddy Mudiam
PLOS ONE.2019; 14(12): e0225129. CrossRef

Regulation of Piplartine on Apoptotic Death of Human Prostate Cancer Cells: Based on Microarray: Eun Hee Kong, Kwang Youn Kim, Sun Nyoung Yu, Sun Yi Lee, Soon Cheol Ann; Kosin Med J. 2010;25(2):117-122. Published online December 31, 2010

136 View
0 Download

PDF

Effective Cancer Therapy : Effect of TRAIL-induced Apoptosis: Young Ho Kim, Jeong Hoon Heo; Kosin Med J. 2010;25(1):1-9. Published online June 30, 2010

224 View
3 Download

PDF

Cannabinoid Protects Primary Cortical Neurons from Apoptosis by Chronic Hypoxia: Ho Chan Kim; Kosin Med J. 2009;24(1):23-27. Published online June 30, 2009

173 View
0 Download

PDF

Organic Hyrdroperdoxide-Induced Cell Death in Renal Epithelial Cells: Geun Tae Kim; Kosin Med J. 2009;24(1):87-94. Published online June 30, 2009

178 View
0 Download

PDF

Mithramycin a Induces Apoptosis and Potentiates Anti-tumor Activity of Cisplatin in Head and Neck Cancer Cell Line: Kang Dae Lee, Seong Hwan Sohn, Jong Wook Park, Young Rok Kim, Bong Ju Lee, Joo Yeun Kim; Kosin Med J. 2008;23(1):1-8. Published online March 31, 2008

181 View
0 Download

PDF

Apoptosis by Cyanate in Kidney Mesangial Cell: Ji Yeol Kim, Yang Soo Kim, Ki Yeon Hong, Wan Kyu Eo, Young Sik Choi; Kosin Med J. 2007;22(1):160-169. Published online June 30, 2007

155 View
0 Download

PDF

The Effect of Apoptosis Related Protein Expression by Cyclosporine in Osteoblast: Young Lim Oh, Wan Kyu Eo, Ki Yeon Hong, Yang Soo Kim, Heung Yeol Kim; Kosin Med J. 2007;22(1):232-239. Published online June 30, 2007

172 View
0 Download

PDF

APOPTOSIS: Myung-Woong Chang; The Journal of Kosin Medical College. 1995;10(2):9-16.

219 View
2 Download

PDF

Effect of Oxygenation on Radiation-Induced Apoptosis of Human Osteosarcoma Cell Line (Saos-2): Jae Do Kim, Gu Hee Jung, Jung Hwan Son, Jung Ho Park, Jae Ho Jang, Young Ho Kwon, Myung Rae Cho, So Hak Jung; Kosin Med J. 2001;16(1):54-61.

157 View
0 Download

PDF

Search