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Hyun Yong Hwang 8 Articles
Degree of Agreement between Phadia EliA ENA and Euroimmun line Immunoassay; Comparison of Two Methods to Evaluate the Ability to Detect ENA Antibodies
Hyun Yong Hwang
Kosin Med J. 2012;27(1):25-30.   Published online June 11, 2012
DOI: https://doi.org/10.7180/kmj.2012.27.1.25
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Abstract PDFPubReader   ePub   CrossRef-TDMCrossref - TDM
Objectives

The purpose of this study is to compare newly developed assay for identification of ENA antibody, Phadia EliA ENA with Euroimmun line immunoassay by analyzing the degree of agreement and the individual antibodies between two methods.

Methods

A total of 82 patient samples were used. Indirect immunofluorescence assay using Hep-2 cell was performed to screen the antinuclear antibody (ANA). Euroimmun line immunoassay (LIA) and Phadia EliA ENA assay were tested to identify the antibodies against extractable nuclear antigens (ENAs). Kappa statistics was used to evaluate the degree of agreement.

Results

Mean age of patients was 41.0 (8-79), and the M:F ratio was 21:61. ANA was positive in 74 samples, and negative were 8 samples. Kappa analysis of the 82 tested samples showed a moderate strength of agreement (κ = 0.521, P = 0.000). There were differences in the order of identified individual antibodies between two methods (Ro > La = RNP > Centromere > Sm > Scl-70 in Phadia EliA ENA, Ro > RNP > Sm>La > Scl-70 > Centromere=Jo-1 in Euroimmun LIA). Ro antibody was most frequently identified in Phadia EliA ENA negative-Euroimmun LIA positive specimens (Ro > RNP = Jo-1 > La = Sm = Centromere > Scl-70).

Conclusions

A moderate strength of agreement was observed between the Phadia EliA ENA and the Euroimmun LIA. There seemed to be a significant difference in the ratio of individual antibodies, especially in the anti-Ro and Sm antibodies.

Simplified Chequerboard Assay for the Evaluation of Anti-Cancer Combination Chemotherapy
Hyun Yong Hwang
Kosin Med J. 2010;25(2):38-43.   Published online December 31, 2010
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The More Fun They Have, The More They Learn?; The Analysis of Relationship between Academic Accomplishment and Teaching Patterns
Hyun Yong Hwang
Kosin Med J. 2008;23(4):132-136.   Published online December 31, 2008
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Effect Determination of Anti-tuberculosis Combination Chemotherapy By Chequerboard Assay
Hyun Yong Hwang, Il Kwon Bae, You Nae Lee, Seok Hoon Jeong
Kosin Med J. 2007;22(2):126-131.   Published online December 31, 2007
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Analysis of Hematologic Parameter Changes in Delayed Samples on the Beckman Coulter LH750 Hematologic Analyzer
Hyun Yong Hwang, Mi Hyang Kim
Kosin Med J. 2007;22(1):49-57.   Published online June 30, 2007
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Escherichia coli Producing CTX-M Extended-Spectrum βᅳLactamase
Hyun Yong Hwang, Jae Sun Park, Il Kwon Bae, Yu Nae Lee, Seok Hook Jeong
Kosin Med J. 2007;22(1):58-67.   Published online June 30, 2007
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Immunophenotypic analysis of Bone Marrow Cells in Children with Acute Lymphoblastic Leukemia
Hyun Yong Hwang, Mi Hyang Kim, Yoo Rha Hong, Jae Sun Park
Kosin Med J. 2006;21(2):45-50.
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KMJ : Kosin Medical Journal